TY  - Data
T1  - Flavonoid metabolism data of Sammi (2020)
A1  - Ma Xiaofei
A1  - Yin Xiaoyue
A1  - chaojuqian
DO  - 10.12072/ncdc.ZDYF.db1681.2022
PY  - 2022
DA  - 2022-02-18
PB  - National Cryosphere Desert Data Center
AB  - The Shami sample is dried in an oven at 55 ℃ for 24 hours, crushed, accurately weighed, 0.05 g of powder is placed in a 2.0 ml Eppendorf tube, added with 1.5 ml of methanol, weighed and stored overnight. Ultrasonic extraction at 40 ℃ for 30 min (SB-800 DTD ultrasound, Ningbo Xinzhi Biotechnology Co., Ltd.; power: 100 W; frequency: 40 kHz); Cool down and add methanol to make up to the initial weight. Mix at 12000 × G centrifugation for 10 min, after 0.22 μ M filter (Pall, MI, USA), take the supernatant as the test solution for uplc-qq-ms analysis. When equipped with acquity UPLC beh C18 column (100 mm × 2.1 mm，1.8  μ m) The chromatographic separation of was performed by acquity uplctm I-class system (waters, Milford, USA). The chromatographic column is acquity UPLC beh C18 column (100 mm × 2.1 mm，1.8  μ m) The mobile phases were 0.1% formic acid aqueous solution (a) and 0.1% formic acid acetonitrile solution (b); Elution gradient: 0 min, 5% B; 1 min，5%B； 3.5 min，30%B； 5.5 min, 60% B. flow rate 0.50 ml · min − 1, column temperature 40 ℃, injection volume 1.0 μ 50. Ab SCIEX API 6500 triple quadrupole mass spectrometer (Applied Biosystems, Toronto, Canada) was used for mass spectrometry. Electrospray ion source (ESI), negative ion mode; The quantitative analysis was based on multiple reaction monitoring (MRM), with ion source temperature of 550 C and spray voltage of -4500V. Retention time (TR), quantitative ion pair, clustering potential (DP), collision energy (CE) and cell exit potential (CXP) values of each analytical component.
DB  - NCDC
UR  - http://www.ncdc.ac.cn/portal/metadata/f3390ee8-6a97-4e96-999d-4466fa5ac786
ER  -