根据林先贵等《土壤微生物研究原理与方法》结合本项目的要求，制定了统一的取样规范和方法进行取样。在毛乌素沙地，选择同年种植，具有代表性的三种固沙灌木林地，沙蒿、沙柳和柠条林地，每种灌木林地中随机设置3个20 m×20m的样地，各样地间距为20m，同时，在距离灌木林地小于20m处选择裸沙地作为对照。\n
选择在植物生长最旺盛和微生物活动最活跃的8月采取土壤样品。在每个样地中，随机选取5株（丛）灌木，使用直径为2.5cm的灭菌土钻，分别采取灌木下和灌木间0-10cm 深度范围内的土壤，采集样品前去除表层植物枯落物。将每个样地采集的土样按灌木下和灌木间土样混合为一个混合样（约1000g），装入无菌塑料袋，保存于保温箱中，并迅速运回实验站，将混合后的土样过2mm 筛以去除根系和枯落物。过筛后的每个土样立即存储在-20℃条件下，用于高通量测序。", "ds_source": "
在毛乌素沙地，选择同年种植，具有代表性的三种固沙灌木林地，沙蒿、沙柳和柠条林地，每种灌木林地中随机设置3个20 m × 20 m的样地，各样地间距为20 m，同时，在距离灌木林地小于20 m处选择裸沙地作为对照。", "ds_process_way": "
选择在植物生长最旺盛和微生物活动最活跃的8月采取土壤样品。在每个样地中，随机选取5株（丛）灌木，使用直径为2.5 cm的灭菌土钻，分别采取灌木下和灌木间0-10 cm 深度范围内的土壤，采集样品前去除表层植物枯落物。将每个样地采集的土样按灌木下和灌木间土样混合为一个混合样（约1000 g），装入无菌塑料袋，保存于保温箱中，并迅速运回实验站，将混合后的土样过2 mm 筛以去除根系和枯落物。过筛后的每个土样立即存储在-20℃条件下，用于高通量测序。", "ds_quality": "
数据质量良好。", "ds_acq_start_time": "2016-01-01 00:00:00", "ds_acq_end_time": "2016-12-31 00:00:00", "ds_acq_place": "宁夏盐池毛乌素沙地生态系统国家定位观测研究站", "ds_acq_lon_east": 107.66666666666667, "ds_acq_lat_south": 37.06666666666667, "ds_acq_lon_west": 106.5, "ds_acq_lat_north": 38.15, "ds_acq_alt_low": null, "ds_acq_alt_high": null, "ds_share_type": "login-access", "ds_total_size": 19010560, "ds_files_count": 2, "ds_format": "excel", "ds_space_res": null, "ds_time_res": "年", "ds_coordinate": "无", "ds_projection": "", "ds_thumbnail": "3b86d62d-2a4f-40a5-89e2-48a78f9ba501.jpg", "ds_thumb_from": 2, "ds_ref_way": "", "paper_ref_way": "", "ds_ref_instruction": "", "ds_from_station": null, "organization_id": "8534e8f7-cbd5-4771-81d6-d524ffde0065", "ds_serv_man": "敏玉芳", "ds_serv_phone": "0931-4967596", "ds_serv_mail": "ncdc@lzb.ac.cn", "doi_value": "10.12072/ncdc.ZDYF.db1679.2022", "subject_codes": [ "170.4510" ], "quality_level": 3, "publish_time": "2022-02-18 14:33:02", "last_updated": "2025-06-30 11:29:08", "protected": false, "protected_to": null, "lang": "zh", "cstr": "11738.11.ncdc.ZDYF.db1679.2022", "i18n": { "en": { "title": "Analysis data of soil bacterial community structure of typical sand fixing shrub land in Maowusu Sandy Land (2016)", "ds_format": "Excel", "ds_source": "
In the Mao Wusu sandland, three representative sand-fixing shrublands, Artemisia spp, Salix spp and Citrus aurantium spp, were selected to be planted in the same year, and three randomly set up sample plots of 20 m × 20 m in each type of shrubland, with the spacing of each sample plot being 20 m. At the same time, bare sandy areas were selected as a control at a distance of less than 20 m from the shrubland.", "ds_quality": "
Data quality is good.", "ds_ref_way": "", "ds_abstract": "
According to Lin Xiangui et al. “Principles and Methods of Soil Microbial Research” combined with the requirements of this project, a unified sampling specification and method were developed for sampling. In the Maowusu sandland, three representative sand-fixing shrublands, Artemisia spp., Salix spp. and Citrus aurantium spp. were selected to be planted in the same year, and three sample plots of 20 m×20 m were randomly set up in each type of shrubland, with a spacing of 20 m between plots, meanwhile, the bare sandland was selected as a control at a distance of less than 20 m from the shrubland.\n
Soil samples were taken in August, when plant growth was most vigorous and microbial activities were most active. In each sample plot, five shrubs were randomly selected, and a sterilized soil auger with a diameter of 2.5 cm was used to take the soil under the shrubs and between the shrubs in the depth range of 0-10 cm, and the surface plant litter was removed before collecting the samples. The soil samples collected from each sample site were mixed into one mixed sample (about 1000g) according to the soil samples under shrubs and among shrubs, put into sterile plastic bags, kept in a heat preservation box, and quickly transported back to the experiment station, and the mixed soil samples were sieved through a 2mm sieve to remove the root system and litter. After sieving, each soil sample was immediately stored at -20°C for high-throughput sequencing.
", "ds_time_res": "年", "ds_acq_place": "Ningxia Yanchi Maowusu Sandland Ecosystem National Positional Observatory Research Station", "ds_space_res": "", "ds_projection": "", "ds_process_way": "Soil samples were taken in August, when plant growth is at its peak and microbial activity is most active. In each sample plot, five shrubs were randomly selected, and a sterilized soil auger with a diameter of 2.5 cm was used to take soil samples from the depth of 0-10 cm under and between the shrubs, and the surface plant litter was removed before collecting the samples. The soil samples collected from each sample site were mixed into a single sample (about 1000 g) from the under-shrub and between-shrub soil samples, placed in sterile plastic bags, stored in an insulated box, and quickly transported back to the experiment station, where the mixed samples were sieved through a 2-mm sieve to remove roots and litter. After sieving, each soil sample was immediately stored at -20°C for high-throughput sequencing.", "ds_ref_instruction": "" } }, "submit_center_id": "ncdc", "data_level": 0, "recommendation_value": 0, "license_type": "https://creativecommons.org/licenses/by/4.0/", "doi_reg_from": "reg_local", "cstr_reg_from": "reg_local", "doi_not_reg_reason": null, "cstr_not_reg_reason": null, "is_paper_in_submitting": false, "belong_to_nieer": false, "ds_topic_tags": [ "毛乌素沙地", "固沙灌木林", "土壤细菌" ], "ds_subject_tags": [ "自然地理学" ], "ds_class_tags": [], "ds_locus_tags": [ "宁夏盐池毛乌素沙地生态系统国家定位观测研究站" ], "ds_time_tags": [ 2016 ], "ds_contributors": [ { "true_name": "张宇清", "email": "zhangyq@bjfu.edu.cn", "work_for": "北京林业大学", "country": "中国" }, { "true_name": "孙延菲", "email": "syf6046@163.com", "work_for": "北京林业大学", "country": "中国" } ], "ds_meta_authors": [ { "true_name": "张宇清", "email": "zhangyq@bjfu.edu.cn", "work_for": "北京林业大学", "country": "中国" } ], "ds_managers": [ { "true_name": "敏玉芳", "email": "myf@lzb.ac.cn", "work_for": "中国科学院西北生态环境资源研究院", "country": "中国" } ], "category": "沙漠与荒漠化" }