本研究试图评估LINC00511在HCC进展中的作用及其机制。方法TCGA和GEO数据库充当支持者，为我们提供临床样本数据。采用Kaplan-Meier法作总体生存分析。用5株细胞系检测LINC00511的表达水平，CCK-8细胞计数试剂盒( CCK-8 )、集落形成法和transwell法检测对细胞行为的影响。通过荧光素酶报告基因检测证实LINC00511与miR-195或眼缺同源物1(EYA1)的相关性。定量实时定量PCR和Western blotting检测mRNA和蛋白表达水平。本研究发现，LINC00511在HCC组织样本和细胞系中高表达，可能与HCC患者不良预后和临床参数有关。功能缺失实验表明，LINC00511缺失抑制HepG2细胞增殖、集落形成和侵袭活性，而功能增益实验显示Huh7细胞有对抗作用。生物信息学工具和荧光素酶报告基因检测发现，LINC00511可能作为miR-195的竞争内源性RNA(ceRNA)，与EYA1呈正相关，拯救实验加强了这一作用。综上所述，LINC00511与EYA1相互作用，通过介导miR-195促进HCC的发生发展，为HCC的诊断和预后提供了一个前景的治疗标志物。
", "ds_source": "采用Kaplan-Meier法作总体生存分析。用5株细胞系检测LINC00511的表达水平，CCK-8细胞计数试剂盒( CCK-8 )、集落形成法和transwell法检测对细胞行为的影响。
", "ds_process_way": "采用Kaplan-Meier法作总体生存分析。用5株细胞系检测LINC00511的表达水平，CCK-8细胞计数试剂盒( CCK-8 )、集落形成法和transwell法检测对细胞行为的影响。通过荧光素酶报告基因检测证实LINC00511与miR-195或眼缺同源物1(EYA1)的相关性。定量实时定量PCR和Western blotting检测mRNA和蛋白表达水平。
", "ds_quality": "数据质量良好
", "ds_acq_start_time": "2017-07-01 00:00:00", "ds_acq_end_time": "2019-12-31 00:00:00", "ds_acq_place": "甘肃兰州", "ds_acq_lon_east": null, "ds_acq_lat_south": null, "ds_acq_lon_west": null, "ds_acq_lat_north": null, "ds_acq_alt_low": null, "ds_acq_alt_high": null, "ds_share_type": "apply-access", "ds_total_size": 9776720, "ds_files_count": 2, "ds_format": "pdf", "ds_space_res": null, "ds_time_res": "", "ds_coordinate": "无", "ds_projection": "", "ds_thumbnail": "50e200d7-cac7-43c4-bd3e-6918e445d2f5.png", "ds_thumb_from": 0, "ds_ref_way": "", "paper_ref_way": "", "ds_ref_instruction": "", "ds_from_station": null, "organization_id": "9971252d-7beb-4464-bc08-bdcc5a1d7dd1", "ds_serv_man": "敏玉芳", "ds_serv_phone": "0931-4967596", "ds_serv_mail": "ncdc@lzb.ac.cn", "doi_value": "", "subject_codes": [], "quality_level": 3, "publish_time": "2023-07-19 11:00:36", "last_updated": "2023-07-21 02:10:04", "protected": false, "protected_to": null, "lang": "zh", "cstr": "11738.11.NCDC.IMP.DB2899.2023", "i18n": { "en": { "title": "LINC00511 as a ceRNA promotes cell malignant behaviors and correlates with prognosis of hepatocellular carcinoma patients by modulating miR-195/EYA1 axis", "ds_format": "", "ds_source": "Kaplan Meier method was used for overall survival analysis. The expression level of linc00511 was detected by 5 cell lines. CCK-8 cell counting Kit (CCK-8), colony forming assay and Transwell assay were used to detect the effect of linc00511 on cell behavior.
", "ds_quality": "Good data quality
", "ds_ref_way": "", "ds_abstract": "This study aims to evaluate the role and mechanism of linc00511 in HCC progression. Methods TCGA and geo database served as supporters to provide us with clinical sample data. Kaplan Meier method was used for overall survival analysis. The expression level of linc00511 was detected by 5 cell lines. CCK-8 cell counting Kit (CCK-8), colony forming assay and Transwell assay were used to detect the effect of linc00511 on cell behavior. The correlation between linc00511 and miR-195 or Eya1 was confirmed by luciferase reporter gene detection. The mRNA and protein expression levels were detected by quantitative real-time PCR and Western blotting. This study found that linc00511 was highly expressed in HCC tissue samples and cell lines, which may be related to poor prognosis and clinical parameters of HCC patients. Loss of function assay showed that linc00511 deletion inhibited the proliferation, colony formation and invasion of HepG2 cells, while functional gain assay showed that Huh7 cells had antagonistic effect. Bioinformatics tools and luciferase reporter gene detection showed that linc00511 may be a competitive endogenous RNA (Cerna) of miR-195, which was positively correlated with Eya1, and rescue experiment strengthened this role. In conclusion, linc00511 interacts with Eya1 to promote the occurrence and development of HCC by mediating miR-195, which provides a promising therapeutic marker for the diagnosis and prognosis of HCC.
", "ds_time_res": "", "ds_acq_place": "", "ds_space_res": "", "ds_projection": "", "ds_process_way": "", "ds_ref_instruction": "" } }, "submit_center_id": "ncdc", "data_level": 0, "recommendation_value": 0, "license_type": "https://creativecommons.org/licenses/by/4.0/", "doi_reg_from": "reg_local", "cstr_reg_from": "reg_local", "doi_not_reg_reason": null, "cstr_not_reg_reason": null, "is_paper_in_submitting": false, "belong_to_nieer": false, "ds_topic_tags": [ "肝癌", "预后", "免疫", "分子检测" ], "ds_subject_tags": [], "ds_class_tags": [], "ds_locus_tags": [ "兰州", "甘肃省" ], "ds_time_tags": [ 2017, 2018, 2019 ], "ds_contributors": [ { "true_name": "肖国青", "email": "xiaogq@impcas.ac.cn", "work_for": "中国科学院近代物理研究所", "country": "中国" } ], "ds_meta_authors": [ { "true_name": "肖国青", "email": "xiaogq@impcas.ac.cn", "work_for": "中国科学院近代物理研究所", "country": "中国" } ], "ds_managers": [ { "true_name": "肖国青", "email": "xiaogq@impcas.ac.cn", "work_for": "中国科学院近代物理研究所", "country": "中国" } ], "category": "其他" }