沙米样品55℃烘箱干燥24小时，粉碎，精确称量0.05 g粉末置2.0 mL Eppendorf管中，加入1.5 mL甲醇，称重，过夜。40℃下超声提取30 min（SB-800 DTD超声仪，宁波新芝生物科技股份有限公司；功率：100 W；频率：40 kHz）；放冷，加入甲醇补充至初始重量。将混合物于12000×g离心10 min，经0.22 μm过滤器（PALL, MI, USA）过滤，取上清液作为供试品溶液，进行UPLC-QQQ-MS分析。在配备ACQUITY UPLC BEH C18色谱柱（100 mm×2.1 mm，1.8 μm）的色谱分离采用ACQUITY UPLCTM I-Class系统（Waters，Milford，USA）。色谱柱为ACQUITY UPLC BEH C18色谱柱（100 mm×2.1 mm，1.8 μm），流动相为0.1%甲酸水溶液（A）和0.1%甲酸乙腈溶液（B）；洗脱梯度：0 min，5%B；1 min，5%B；3.5 min，30%B；5.5 min，60%B. 流速0.50 mL·min−1，柱温40℃，进样量1.0 μL.质谱分析采用AB Sciex API 6500三重四极杆质谱仪（Applied Biosystems，Toronto，Canada）。电喷雾离子源（ESI），负离子模式；定量分析采用多反应监测模式（MRM），离子源温度550℃，喷雾电压-4500V。各分析成分的保留时间（tR）、定量离子对、去簇电压（declustering potential, DP）、碰撞能量（collision energy, CE）和出口电压（cell exit potential, CXP）值。", "ds_source": "
沙米样品55℃烘箱干燥24小时，粉碎，精确称量0.05 g粉末置2.0 mL Eppendorf管中，加入1.5 mL甲醇，称重，过夜。40℃下超声提取30 min（SB-800 DTD超声仪，宁波新芝生物科技股份有限公司；功率：100 W；频率：40 kHz）；放冷，加入甲醇补充至初始重量。将混合物于12000×g离心10 min，经0.22 μm过滤器（PALL, MI, USA）过滤，取上清液作为供试品溶液，进行UPLC-QQQ-MS分析。在配备ACQUITY UPLC BEH C18色谱柱（100 mm×2.1 mm，1.8 μm）的色谱分离采用ACQUITY UPLCTM I-Class系统（Waters，Milford，USA）。色谱柱为ACQUITY UPLC BEH C18色谱柱（100 mm×2.1 mm，1.8 μm），流动相为0.1%甲酸水溶液（A）和0.1%甲酸乙腈溶液（B）；洗脱梯度：0 min，5%B；1 min，5%B；3.5 min，30%B；5.5 min，60%B. 流速0.50 mL·min−1，柱温40℃，进样量1.0 μL.质谱分析采用AB Sciex API 6500三重四极杆质谱仪（Applied Biosystems，Toronto，Canada）。电喷雾离子源（ESI），负离子模式；定量分析采用多反应监测模式（MRM），离子源温度550℃，喷雾电压-4500V。各分析成分的保留时间（tR）、定量离子对、去簇电压（declustering potential, DP）、碰撞能量（collision energy, CE）和出口电压（cell exit potential, CXP）值。", "ds_process_way": "
实验室沙米样品干燥、粉碎、称重、过夜、超声提取等一系列处理后；进行UPLC-QQQ-MS分析、质谱分析、定量分析。", "ds_quality": "
数据质量良好。", "ds_acq_start_time": "2020-01-01 00:00:00", "ds_acq_end_time": "2020-12-31 00:00:00", "ds_acq_place": "北方半干旱区", "ds_acq_lon_east": null, "ds_acq_lat_south": null, "ds_acq_lon_west": null, "ds_acq_lat_north": null, "ds_acq_alt_low": null, "ds_acq_alt_high": null, "ds_share_type": "login-access", "ds_total_size": 71743, "ds_files_count": 2, "ds_format": "excel", "ds_space_res": null, "ds_time_res": "", "ds_coordinate": "无", "ds_projection": "", "ds_thumbnail": "933c3283-f319-41ed-a4e8-c3a3fa890f1f.png", "ds_thumb_from": 2, "ds_ref_way": "", "paper_ref_way": "", "ds_ref_instruction": "", "ds_from_station": null, "organization_id": "8534e8f7-cbd5-4771-81d6-d524ffde0065", "ds_serv_man": "敏玉芳", "ds_serv_phone": "0931-4967596", "ds_serv_mail": "ncdc@lzb.ac.cn", "doi_value": "10.12072/ncdc.ZDYF.db1677.2022", "subject_codes": [ "170.4510" ], "quality_level": 3, "publish_time": "2022-02-18 14:33:08", "last_updated": "2025-06-30 11:29:09", "protected": false, "protected_to": null, "lang": "zh", "cstr": "11738.11.ncdc.ZDYF.db1677.2022", "i18n": { "en": { "title": "Data on organic acid metabolism in sago (2020)", "ds_format": "excel", "ds_source": "
Sarmi samples were oven-dried at 55°C for 24 h, pulverized, accurately weighed 0.05 g of powder placed in a 2.0 mL Eppendorf tube, 1.5 mL of methanol was added, weighed, and extracted by ultrasonication at 40°C for 30 min (SB-800 DTD ultrasonator, Ningbo Xinzhi Bio-technology Co., Ltd; power: 100 W; Frequency: 40 kHz); let cool and add methanol to replenish to the initial weight. The mixture was centrifuged at 12000×g for 10 min, filtered through a 0.22 μm filter (PALL, MI, USA), and the supernatant was taken as the test solution for UPLC-QQQ-MS analysis. Chromatographic separation on an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.8 μm) equipped with ACQUITY UPLC was performed using an ACQUITY UPLCTM I-Class system (Waters, Milford, USA). The chromatographic column was an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.8 μm), and the mobile phases were 0.1% formic acid aqueous solution (A) and 0.1% formic acid acetonitrile solution (B); the elution gradient was: 0 min, 5% B; 1 min, 5% B; 3.5 min, 30% B; 5.5 min, 60% B. The flow rate was 0.50 mL -min-1, column temperature 40°C, injection volume 1.0 μL.Mass spectrometry analysis was performed on an AB Sciex API 6500 triple quadrupole mass spectrometer (Applied Biosystems, Toronto, Canada). The mass spectrometry analysis was performed on an AB Sciex API 6500 triple quadrupole mass spectrometer (Applied Biosystems, Toronto, Canada) with an electrospray ionization (ESI) source in negative ionization mode, and the quantitative analysis was performed in multi-reaction monitoring (MRM) mode, with an ionization source temperature of 550°C and a spray voltage of -4500 V. The retention times (tR), quantitative ion pairs, declustering potential (DP), collision energy , CE) and cell exit potential (CXP) values for each analyzed component.", "ds_quality": "
Data quality is good.", "ds_ref_way": "", "ds_abstract": "
Sarmi samples were oven-dried at 55°C for 24 h, pulverized, accurately weighed 0.05 g of powder placed in a 2.0 mL Eppendorf tube, 1.5 mL of methanol was added, weighed, and extracted by ultrasonication at 40°C for 30 min (SB-800 DTD ultrasonator, Ningbo Xinzhi Bio-technology Co., Ltd; power: 100 W; Frequency: 40 kHz); let cool and add methanol to replenish to the initial weight. The mixture was centrifuged at 12000×g for 10 min, filtered through a 0.22 μm filter (PALL, MI, USA), and the supernatant was taken as the test solution for UPLC-QQQ-MS analysis. Chromatographic separation on an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.8 μm) equipped with ACQUITY UPLC was performed using an ACQUITY UPLCTM I-Class system (Waters, Milford, USA). The chromatographic column was an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.8 μm), and the mobile phases were 0.1% formic acid aqueous solution (A) and 0.1% formic acid acetonitrile solution (B); the elution gradient was: 0 min, 5% B; 1 min, 5% B; 3.5 min, 30% B; 5.5 min, 60% B. The flow rate was 0.50 mL -min-1, column temperature 40°C, injection volume 1.0 μL.Mass spectrometry analysis was performed on an AB Sciex API 6500 triple quadrupole mass spectrometer (Applied Biosystems, Toronto, Canada). The mass spectrometry analysis was performed on an AB Sciex API 6500 triple quadrupole mass spectrometer (Applied Biosystems, Toronto, Canada) with an electrospray ionization source (ESI) in negative ionization mode, and the quantitative analysis was performed in multi-reaction monitoring (MRM) mode, with an ionization source temperature of 550 ℃ and a spray voltage of -4500 V. The retention times of the analyzed components (tR), the quantified ion pairs, the de-clustering potential (DP), collision energy , CE) and cell exit potential (CXP) values for each analyzed component.
", "ds_time_res": "", "ds_acq_place": "Northern semi-arid region", "ds_space_res": "", "ds_projection": "", "ds_process_way": "After a series of treatments such as drying, pulverizing, weighing, overnight, and ultrasonic extraction of laboratory samples of sago; UPLC-QQQ-MS analysis, mass spectrometry, and quantitative analysis were performed.", "ds_ref_instruction": "" } }, "submit_center_id": "ncdc", "data_level": 0, "license_type": "CC BY 4.0", "doi_reg_from": "reg_local", "cstr_reg_from": "reg_local", "doi_not_reg_reason": null, "cstr_not_reg_reason": null, "is_paper_in_submitting": false, "ds_topic_tags": [ "沙米", "色谱柱", "三重四极杆质谱仪", "半干旱" ], "ds_subject_tags": [ "自然地理学" ], "ds_class_tags": [], "ds_locus_tags": [ "北方半干旱区" ], "ds_time_tags": [ 2020 ], "ds_contributors": [ { "true_name": "马小飞", "email": "maxiaofei@lzb.ac.cn", "work_for": "中国科学院西北生态环境资源研究院", "country": "中国" }, { "true_name": "尹晓月", "email": "yxyjing@126.com", "work_for": "中国科学院西北生态环境资源研究院", "country": "中国" }, { "true_name": "钱朝菊", "email": "chaojuqian@lzb.ac.cn", "work_for": "中国科学院西北生态环境资源研究院", "country": "中国" } ], "ds_meta_authors": [ { "true_name": "马小飞", "email": "maxiaofei@lzb.ac.cn", "work_for": "中国科学院西北生态环境资源研究院", "country": "中国" } ], "ds_managers": [ { "true_name": "敏玉芳", "email": "myf@lzb.ac.cn", "work_for": "中国科学院西北生态环境资源研究院", "country": "中国" } ], "category": "沙漠与荒漠化" }