根据林先贵等《土壤微生物研究原理与方法》结合本项目的要求，制定了统一的取样规范和方法进行取样。在毛乌素沙地，选择同年种植，具有代表性的三种固沙灌木林地，沙蒿、沙柳和柠条林地，每种灌木林地中随机设置3个20m×20m的样地，各样地间距为20m，同时，在距离灌木林地小于20m处选择裸沙地作为对照。\n
选择在植物生长最旺盛和微生物活动最活跃的8月采取土壤样品。在每个样地中，随机选取5株（丛）灌木，使用直径为2.5cm 的灭菌土钻，分别采取灌木下和灌木间0-10cm 深度范围内的土壤，采集样品前去除表层植物枯落物。将每个样地采集的土样按灌木下和灌木间土样混合为一个混合样（约1000g），装入无菌塑料袋，保存于保温箱中，并迅速运回实验站，将混合后的土样过2mm 筛以去除根系和枯落物。过筛后的每个土样立即存储在-20℃条件下，用于高通量测序。", "ds_source": "
在毛乌素沙地，选择同年种植，具有代表性的三种固沙灌木林地，沙蒿、沙柳和柠条林地，每种灌木林地中随机设置3个20m×20m的样地，各样地间距为20m，同时，在距离灌木林地小于20m处选择裸沙地作为对照。", "ds_process_way": "
选择在植物生长最旺盛和微生物活动最活跃的8月采取土壤样品。在每个样地中，随机选取5株（丛）灌木，使用直径为2.5cm 的灭菌土钻，分别采取灌木下和灌木间0-10cm 深度范围内的土壤，采集样品前去除表层植物枯落物。将每个样地采集的土样按灌木下和灌木间土样混合为一个混合样（约1000g），装入无菌塑料袋，保存于保温箱中，并迅速运回实验站，将混合后的土样过2mm 筛以去除根系和枯落物。过筛后的每个土样立即存储在-20℃条件下，用于高通量测序。", "ds_quality": "
数据质量良好。", "ds_acq_start_time": "2016-01-01 00:00:00", "ds_acq_end_time": "2016-12-31 00:00:00", "ds_acq_place": "宁夏盐池毛乌素沙地生态系统国家定位观测研究站", "ds_acq_lon_east": 107.66666666666667, "ds_acq_lat_south": 37.06666666666667, "ds_acq_lon_west": 106.5, "ds_acq_lat_north": 38.15, "ds_acq_alt_low": null, "ds_acq_alt_high": null, "ds_share_type": "login-access", "ds_total_size": 7382016, "ds_files_count": 2, "ds_format": "excel", "ds_space_res": null, "ds_time_res": "年", "ds_coordinate": "无", "ds_projection": "", "ds_thumbnail": "9aeb4fcf-9be1-48ce-8b25-6faa4bf92983.jpg", "ds_thumb_from": 2, "ds_ref_way": "", "paper_ref_way": "", "ds_ref_instruction": "", "ds_from_station": null, "organization_id": "8534e8f7-cbd5-4771-81d6-d524ffde0065", "ds_serv_man": "敏玉芳", "ds_serv_phone": "0931-4967596", "ds_serv_mail": "ncdc@lzb.ac.cn", "doi_value": "10.12072/ncdc.ZDYF.db1678.2022", "subject_codes": [ "170.4510" ], "quality_level": 3, "publish_time": "2022-02-18 14:32:57", "last_updated": "2025-06-30 11:29:09", "protected": false, "protected_to": null, "lang": "zh", "cstr": "11738.11.ncdc.ZDYF.db1678.2022", "i18n": { "en": { "title": "Data on soil fungal community structure analysis of typical sand fixation shrubland in the Maowusu Sandland (2016)", "ds_format": "excel", "ds_source": "
In the Mao Wusu sandland, three representative sand-fixing shrubland planted in the same year, Artemisia spp., Salix spp. and Citrus aurantium spp. were selected, three sample plots of 20m×20m were randomly set up in each type of shrubland, with the spacing of each sample plot being 20m, and at the same time, bare sandy areas were selected at a distance of less than 20m from the shrubland to serve as a control.", "ds_quality": "
Data quality is good.", "ds_ref_way": "", "ds_abstract": "
According to Lin Xiangui et al. “Principles and Methods of Soil Microbial Research” combined with the requirements of this project, a unified sampling specification and method were developed for sampling. In the Maowusu sandland, three representative sand-fixing shrublands, Artemisia spp., Salix spp. and Citrus aurantium spp. were selected to be planted in the same year, and three sample plots of 20m×20m were randomly set up in each type of shrubland, with a spacing of 20m between plots, meanwhile, the bare sandland was selected as a control at a distance of less than 20m from the shrubland.\n
Soil samples were taken in August, when plant growth was most vigorous and microbial activities were most active. In each sample plot, five shrubs were randomly selected, and a sterilized soil auger with a diameter of 2.5 cm was used to take the soil under the shrubs and between the shrubs in the depth range of 0-10 cm, and the surface plant litter was removed before collecting the samples. The soil samples collected from each sample site were mixed into one mixed sample (about 1000g) according to the soil samples under shrubs and among shrubs, put into sterile plastic bags, kept in a heat preservation box, and quickly transported back to the experiment station, and the mixed soil samples were sieved through a 2mm sieve to remove the root system and litter. After sieving, each soil sample was immediately stored at -20°C for high-throughput sequencing.
", "ds_time_res": "年", "ds_acq_place": "Ningxia Yanchi Maowusu Sandland Ecosystem National Positional Observatory Research Station", "ds_space_res": "", "ds_projection": "", "ds_process_way": "Soil samples were taken in August, when plant growth is at its peak and microbial activity is most active. In each sample plot, five shrubs were randomly selected, and a sterilized soil auger with a diameter of 2.5 cm was used to take soil samples from 0-10 cm depth under and between shrubs, and the surface plant litter was removed before sample collection. The soil samples collected from each sample site were mixed into one mixed sample (about 1000g) according to the soil samples under shrubs and between shrubs, put into sterile plastic bags, kept in a heat preservation box, and quickly transported back to the experiment station, and the mixed soil samples were sieved through a 2mm sieve to remove the root system and litter. After sieving, each soil sample was immediately stored at -20°C for high-throughput sequencing.", "ds_ref_instruction": "" } }, "submit_center_id": "ncdc", "data_level": 0, "license_type": "CC BY 4.0", "doi_reg_from": "reg_local", "cstr_reg_from": "reg_local", "doi_not_reg_reason": null, "cstr_not_reg_reason": null, "is_paper_in_submitting": false, "ds_topic_tags": [ "毛乌素沙地", "固沙灌木林", "土壤细菌" ], "ds_subject_tags": [ "自然地理学" ], "ds_class_tags": [], "ds_locus_tags": [ "宁夏盐池毛乌素沙地生态系统国家定位观测研究站" ], "ds_time_tags": [ 2016 ], "ds_contributors": [ { "true_name": "张宇清", "email": "zhangyq@bjfu.edu.cn", "work_for": "北京林业大学", "country": "中国" }, { "true_name": "孙延菲", "email": "syf6046@163.com", "work_for": "北京林业大学", "country": "中国" } ], "ds_meta_authors": [ { "true_name": "张宇清", "email": "zhangyq@bjfu.edu.cn", "work_for": "北京林业大学", "country": "中国" } ], "ds_managers": [ { "true_name": "敏玉芳", "email": "myf@lzb.ac.cn", "work_for": "中国科学院西北生态环境资源研究院", "country": "中国" } ], "category": "沙漠与荒漠化" }