Sarmi samples were oven dried at 55°C for 24 h, pulverized, accurately weighed 0.05 g of powder placed in a 2.0 mL Eppendorf tube, 1.5 mL of methanol was added, weighed, and extracted by ultrasonication at 40°C for 30 min (SB-800 DTD ultrasonator, Ningbo Xinzhi Bio-technology Co., Ltd; power: 100 W; Frequency: 40 kHz); let cool and add methanol to replenish to the initial weight. The mixture was centrifuged at 12000×g for 10 min, filtered through a 0.22 μm filter (PALL, MI, USA), and the supernatant was taken as the test solution for UPLC-QQQ-MS analysis. Chromatographic separation on an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.8 μm) equipped with ACQUITY UPLC was performed using an ACQUITY UPLCTM I-Class system (Waters, Milford, USA). The chromatographic column was an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.8 μm), and the mobile phases were 0.1% formic acid aqueous solution (A) and 0.1% formic acid acetonitrile solution (B); the elution gradient was: 0 min, 5% B; 1 min, 5% B; 3.5 min, 30% B; 5.5 min, 60% B. The flow rate was 0.50 mL -min-1, column temperature 40°C, injection volume 1.0 μL.Mass spectrometry analysis was performed on an AB Sciex API 6500 triple quadrupole mass spectrometer (Applied Biosystems, Toronto, Canada). The mass spectrometry analysis was performed on an AB Sciex API 6500 triple quadrupole mass spectrometer (Applied Biosystems, Toronto, Canada) with an electrospray ionization (ESI) source in negative ionization mode, and the quantitative analysis was performed in multi-reaction monitoring (MRM) mode, with an ionization source temperature of 550°C and a spray voltage of -4500 V. The retention times (tR), quantitative ion pairs, declustering potential (DP), collision energy , CE) and cell exit potential (CXP) values for each analyzed component.
Sarmi samples were oven-dried at 55°C for 24 h, pulverized, accurately weighed 0.05 g of powder placed in a 2.0 mL Eppendorf tube, 1.5 mL of methanol was added, weighed, and extracted by ultrasonication at 40°C for 30 min (SB-800 DTD ultrasonator, Ningbo Xinzhi Bio-technology Co., Ltd; power: 100 W; Frequency: 40 kHz); let cool and add methanol to replenish to the initial weight. The mixture was centrifuged at 12000×g for 10 min, filtered through a 0.22 μm filter (PALL, MI, USA), and the supernatant was taken as the test solution for UPLC-QQQ-MS analysis. Chromatographic separation on an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.8 μm) equipped with ACQUITY UPLC was performed using an ACQUITY UPLCTM I-Class system (Waters, Milford, USA). The chromatographic column was an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.8 μm), and the mobile phases were 0.1% formic acid aqueous solution (A) and 0.1% formic acid acetonitrile solution (B); the elution gradient was: 0 min, 5% B; 1 min, 5% B; 3.5 min, 30% B; 5.5 min, 60% B. The flow rate was 0.50 mL -min-1, column temperature 40°C, injection volume 1.0 μL.Mass spectrometry analysis was performed on an AB Sciex API 6500 triple quadrupole mass spectrometer (Applied Biosystems, Toronto, Canada). The mass spectrometry analysis was performed on an AB Sciex API 6500 triple quadrupole mass spectrometer (Applied Biosystems, Toronto, Canada) with an electrospray ionization source (ESI) in negative ionization mode, and the quantitative analysis was performed in multi-reaction monitoring (MRM) mode, with an ionization source temperature of 550°C and a spray voltage of -4500 V. The retention times of the analyzed components (tR), the quantified ion pairs, the de-clustering potential (DP), collision energy , CE) and cell exit potential (CXP) values for each analyzed component.
Laboratory samples of sago were dried, pulverized, weighed, overnight, and ultrasonically extracted in a series of treatments; UPLC-QQQ-MS analysis, mass spectrometry, and quantitative analysis were performed.
Data quality is good.
| # | number | name | type |
| 1 | 2016YFC0500900 | Key technologies and demonstrations for desertification control in semi-arid desert areas of northern China | National key R & D plan |
This work is licensed under
CC BY 4.0 (Creative Commons Attribution 4.0 International License).
CSTR:11738.11.ncdc.ZDYF.db1681.2022
10.12072/ncdc.ZDYF.db1681.2022
